[Đồ án tốt nghiệp bằng tiếng Anh] Effect of material treatment methods on recovery yield, quality parameters and antimicrobial activities of essential oil obtained from turmeric leaf using hydrodistillation

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TABLE OF CONTENTS
ACKNOWLEDGEMENTS...........................................................................................................  iii
DISCLAIMER  ................................................................................................................................  iv
LIST OF FIGURES  ........................................................................................................................  ix
LIST OF TABLES...........................................................................................................................  x
LIST OF ACRONYMS  ..................................................................................................................  xi
ABSTRACT  ................................................................................................................................. xii
Chapter 1:  INTRODUCTION  ...................................................................................................  1
1.1.  Introduction ..........................................................................................................................  1
1.2.  Research content  ...................................................................................................................  1
1.3.  Research aims  .......................................................................................................................  2
1.4.  Overall experimental design of the project  ...........................................................................  2
1.5.  Research hypotheses  .............................................................................................................  2
Chapter 2: LITERATURE REVIEW..........................................................................................  4
2.1.  Overview of turmeric and turmeric leaves  ...........................................................................  4
2.1.1.  Origin ............................................................................................................................  4
2.1.2.  Characteristic  .................................................................................................................  4
2.1.3.  Chemical composition and application  .........................................................................  8
2.1.3.1.  Chemical composition  ...........................................................................................  8
2.1.3.2.  Nutritional value  ..................................................................................................  10
2.1.3.3.  Application  ...........................................................................................................  10
2.2.  Overview of essential oils...................................................................................................  12
2.2.1.  Definition ....................................................................................................................  12
2.2.2.  Origin and development of essential oils  ....................................................................  13
2.2.3.  Physical properties and chemical compositions of essential oils  ................................  13
2.2.3.1.  Physical properties  ...............................................................................................  14
2.2.3.2.  Chemical compositions  ........................................................................................  14
2.2.4.  Applications of essential oils  .......................................................................................  17
2.3.  Techniques for recovering essential oils ............................................................................  18
2.3.1.  Distillation methods  ....................................................................................................  18
2.3.1.1.  Hydro-distillation (HD)  .......................................................................................  18
2.3.1.2.  Water-steam distillation  .......................................................................................  18
2.3.1.3.  Steam distillation (SD)  .........................................................................................  19
2.3.2.  Solvent extraction  ........................................................................................................  19
2.3.3.  Cold pressing  ...............................................................................................................  20 
vi
2.3.4.  Soxhlet extraction  ........................................................................................................  21
2.3.5.  New methods in essential oil extraction  ......................................................................  22
2.3.5.1.  Microwave-Assisted Hydro-distillation (MAHD)  ...............................................  22
2.3.5.2.  Ultrasound-Assisted extraction (UAE)  ................................................................  23
2.3.5.3.  Supercritical Fluid Extraction (SFE)  ...................................................................  23
2.4.2.  The main influencing factors in steam distillation  ......................................................  25
2.4.2.1.  Diffusion  ..............................................................................................................  25
2.4.2.2.  Hydrolysis  ............................................................................................................  26
2.4.2.3.  Temperature  .........................................................................................................  26
2.4.2.4.  Advantages and disadvantages ............................................................................  26
2.5.  Introduction to gas chromatography with mass spectrometry (GC/MS)  ............................  27
2.5.1.  Gas Chromatography (GC)  .........................................................................................  27
2.5.2.  Mass Spectrometry (MS) ............................................................................................  28
2.5.3.  Gas chromatography with mass spectrometry (GC–MS) ...........................................  28
2.6.  A brief overview of pathogenic microorganisms in the research  .......................................  28
2.6.1.  Staphylococcus aureus  ................................................................................................  28
2.6.2.  Escherichia coli  ...........................................................................................................  29
2.7.  Research situation of turmeric leaf essential oil  .................................................................  31
2.7.1.  Domestic studies on turmeric leaf essential oil  ...........................................................  31
2.7.2.  Overseas studies on turmeric leaf essential oil  ............................................................  31
2.7.2.1.  Constituents of the Leaf Oil of Curcuma longa L. from Uttaranchal  ..................  31
2.7.2.2.  Chemical composition and antifungal activity of oil extracted from turmeric 
leaves (Curcuma longa L.)  .....................................................................................................  31
Chapter 3:  RESEARCH MATERIALS AND METHODS  ...................................................  33
3.1.  Plant material  ......................................................................................................................  33
3.2.  Tools, equipment, chemical substances  ..............................................................................  33
3.2.1.  Tools, equipment  .........................................................................................................  33
3.2.2.  Chemical substances ...................................................................................................  33
3.3.  Research diagram ...............................................................................................................  34
3.4.  Research methods  ...............................................................................................................  34
3.4.1.  Experimental design  ....................................................................................................  34
3.4.2.  Analytical methods  ......................................................................................................  36
3.4.2.1.  Determination of total essential oil content in the material  .................................  36
3.4.2.2.  Analysis of physicochemical quality  ...................................................................  37
3.4.2.3.  Analysis of chemical composition  .......................................................................  39
3.4.2.4.  Sensory evaluation  ...............................................................................................  39
3.4.2.5.  Analysis of antimicrobial activity  ........................................................................  40
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