[Khóa luận tốt nghiệp] Research on the process of separating collagen from horse skin
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CONTENTS
DECLARATION OF AUTHORSHIP ................................................................... i
ACKNOWLEDGEMENTS .................................................................................. ii
CONTENTS ......................................................................................................... iv
ABBREVIATIONS .............................................................................................. vi
LIST OF TABLES ............................................................................................. viii
LIST OF FIGURES .............................................................................................. ix
PREAMBLE .......................................................................................................... 1
PART I: INTRODUCTION ............................................................................... 3
1.1. What is collagen? ........................................................................................... 3
1.2. How many types of collagen are there? ......................................................... 4
1.3. Structures of collagen ..................................................................................... 6
1.4. Application and origin of collagen types ....................................................... 7
1.4.1. Applications in food technology ................................................................. 7
1.4.2. Applications in medicine and pharmaceuticals ........................................... 9
1.4.3. Applications in the cosmetic industry ....................................................... 11
1.5. Research situation on collagen extraction in the country and in the world . 13
PART II. MATERIALS AND METHODS OF RESEARCH ...................... 18
2.1. Materials ....................................................................................................... 18
2.2. Chemicals, machinery and equipment ......................................................... 18
2.3. Methods of research ..................................................................................... 19
2.3.1. Sample treatment ....................................................................................... 21
2.3.2. Collagen extraction ................................................................................... 22
2.3.3. Preservation of hydrolyzed collagen ......................................................... 26
2.3.4. Quantitative technique of collagen concentration dissolved in extract
solution ................................................................................................................ 27
2.3.5. Data processing ......................................................................................... 29
v
PART III. RESULTS AND DISCUSSION ..................................................... 30
3.1. Effect of enzymes on the concentration of soluble collagen in pepsin ........ 32
3.2. Effect of ultrasonic on the concentration of soluble collagen in pepsin ...... 36
3.3. Effect of temperature on the concentration of soluble collagen in pepsin... 38
3.4. Determination of the efficiency of collagen extraction ............................... 39
3.5. Surface morphology of PSC from horse skin .............................................. 42
CONCLUSIONS ................................................................................................ 43
REFERENCE ...................................................................................................... 44
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