[Đồ án tốt nghiệp bằng tiếng Anh] Effects of capping agent concentration and reaction time on antimicrobial activities of copper nanoparticles (CUNPs)
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TABLE OF CONTENTS
Chapter 1: INTRODUCTION ......................................................................................................... 1
1.1 Rationale………………………………………………………………………1
1.2. Thesis objects ...................................................................................................... 1
1.3. Limits and scope of the study .............................................................................. 2
1.4. Research content .................................................................................................. 2
1.5. The scientific and practical significance of the topic .......................................... 2
1.6. Layout of the report ............................................................................................. 2
Chapter 2: OVERVIEW .................................................................................................................. 3
2.1. Overview of nanotechnology .............................................................................. 3
2.1.1. The concept of nanotechnology ................................................................................... 3
2.1.2. Classification of nanomaterials ................................................................................... 3
2.1.3. Applications of nanotechnology in food ..................................................................... 5
2.2. Overview of copper nanoparticles ....................................................................... 6
2.3. Introduction to copper nanoparticles ................................................................... 6
2.3.1. Methods for synthesizing copper nanoparticles .......................................................... 7
2.3.2. Antibacterial mechanism of copper nanoparticles....................................................... 8
2.4. Capping agent ...................................................................................................... 9
2.5. Escherichia coli ................................................................................................. 11
2.6. The fungus Colletotrichum gloeosporioides ..................................................... 12
Chapter 3: MATERIALS AND RESEARCH METHODS ........................................................... 15
3.1. Materials…..…………………………………………………………………..15
3.2. Research methods .............................................................................................. 15
3.2.1. Research diagram ...................................................................................................... 15
3.2.2. Synthesis method for CuNPs ..................................................................................... 16
3.2.3. Characteristic measurement methods of CuNPs ....................................................... 17
3.2.3.1. UV-VIS spectroscopy ........................................................................................ 17
3.2.3.2. Energy-dispersive X-ray spectroscopy (EDS) analysis ......................................... 17
3.2.3.3. X-ray diffraction spectroscopy (XRD) analysis ................................................ 17
3.2.3.4. Scanning Electron Microscope (SEM) analysis ................................................ 18
3.2.3.5. Transmission electron microscopy (TEM) analysis .......................................... 18
3.2.3.6. Zeta potential (ZP) measurement ...................................................................... 19
3.2.4. Antimicrobial activity evaluation methods................................................................ 20
3.2.4.1. Method to determine the resistance to Escherichia coli .................................... 20
3.2.4.2. Method for determining resistance to mold Colletotrichum gloeosporioides ... 20
3.2.5. Data analysis method ................................................................................................. 21
Chapter 4: RESULTS AND DISCUSSION .................................................................................. 22
4.1. Characterization of CuNPs ................................................................................ 22
4.1.1. Optical characteristics (UV – VIS) ............................................................................ 22
4.1.3. X-ray diffraction of CuNPs (XRD) ........................................................................... 24
4.1.4. SEM, TEM and DLS ................................................................................................. 27
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