ThiNganHang Sách - Tài liệu thi tuyển
30 câu trắc nghiệm Quản trị nguồn nhân lực (Có Đáp án)
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LIST OF CONTENTS
Acknowledgements ................................................................................................ i
COMMITMENT ................................................................................................... ii
LIST OF contents ................................................................................................. iii
LIST OF TABLES ................................................................................................ v
LIST OF FIGURES ............................................................................................. vi
SUMMARY ........................................................................................................ vii
CHAPTER 1. INTRODUCTION ......................................................................... 1
1.1. Introduction .................................................................................................... 1
1.1.2.Objectives .................................................................................................... 2
1.1.3. Requirements .............................................................................................. 2
CHAPTER 2. LITERATURE REVIEW .............................................................. 3
2.1. Genetic resources of rice. ............................................................................... 3
2.1.1. Genetic resources ........................................................................................ 3
2.1.2. Global genetic resources of rice ................................................................. 3
2.1.3. Genetic resources of rice in Vietnam .......................................................... 6
2.1.4. Pigmented rice genetic resources ................................................................ 6
2.2. Pigmented rice ............................................................................................... 7
2.2.1. Properties of pigmented rice ....................................................................... 7
2.2.2. Anthocyanin ................................................................................................ 9
2.2.3. The studies about genetic diversity of rice. .............................................. 14
Chapter 3. Materials and methods. ..................................................................... 16
3.1. Materials ...................................................................................................... 16
3.2. METHODS .................................................................................................. 19
3.2.1. Field layout ............................................................................................... 19
3.2.2. DNA extraction with potasium acetate (CH3
COOK). .............................. 20
3.2.3. PCR reaction: ............................................................................................ 20
3.2.4. Electrophoresis:......................................................................................... 21
3.2.5. Data processing. ........................................................................................ 21
iv
3.2.6. Anthocyanin content measurement ........................................................... 22
Chapter 4. Results and discussions ..................................................................... 24
4.1. The result of the DNA extraction of 30 accessions. .................................... 24
4.3. Analysis the genetic relationships between the studied pigmented rice
samples. .................................................................................................... 27
4.3. Total anthocyanin content ............................................................................ 28
CHAPTER 5. CONCLUSIONS AND SUGGESTIONS ................................... 32
5.1. Conclusions .................................................................................................. 32
5.2. Suggestions .................................................................................................. 32
REFERENTS ...................................................................................................... 34
APPENDIX ......................................................................................................... 42