[Khóa luận tốt nghiệp] Investigation of a simple method for extraction c-phycocyanin from the blue-green microalgae arthrospira platensis

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CONTENTS
CHAPTER 1. INTRODUCTION  .........................................................................  1
1.1. Preface  ...................................................................................................  1
1.2. Objectives .............................................................................................  2
1.3. Requirements  ........................................................................................  2
CHAPTER 2. LITERATURE REVIEW  ..............................................................  3
2.1. An overview of Arthrospira platensis  ..................................................  3
2.1.1. Introduction about Arthrospira platensis  .......................................  3
2.1.2. Taxonomy of Arthrospira platensis  ...............................................  4
2.1.3. Chemical composition of the algae Arthrospira platensis  .............  6
2.1.4.  The applications of Arthrospira platensis  .....................................  9
2.1.4.1. For human health  ................................ ................................ .................   9
2.1.4.2. In the cosmetics industry  ................................ ................................ .   10
2.1.4.3. Some studies show positive results  ................................ .................   10
2.2. An overview of C-phycocyanin  ..........................................................  11
2.2.1. Introduction about C-phycocyanin  ...............................................  11
2.2.2. Characteristics of C-phycocyanin  .................................................  12
2.2.3. Applications of C-phycocyanin  ....................................................  13
2.2.4. Some studies on the use of C-phycocyanin  ..................................  14
2.3. Studies on C-phycocyanin extract in the world and Vietnam  ............  18
2.3.1. Studies on C-phycocyanin extract in the World  ...........................  18
2.3.2. Studies on C-phycocyanin extract in Vietnam  .............................  18
CHAPTER 3: MATERIALS AND EXPERIMENTAL METHODS  ................  20 
iv
3.1. Materials .............................................................................................  20
3.1.1. The origin  ......................................................................................  20
3.1.2. Place and time of experiment  .......................................................  20
3.2. Chemicals and equipments  .................................................................  20
3.3. Research methods  ...............................................................................  21
3.3.1. Extraction method of C-PC  ..........................................................  21
3.3.2. Factors affecting the extraction of C-PC  ......................................  23
3.3.3.  Investigate  the  method  of  processing  C-PC  solution  into  dry 
powder  ....................................................................................................  24
3.3.3.1.  Treatment  of  C-PC  solution  into  dry  powder  by  freeze-drying 
method  ................................ ................................ ................................ ...................   24
3.3.3.2.  Treatment  of  C-PC  solution  into  dry  powder  by  natural 
evaporation method  ................................ ................................ ..............................   25
3.3.4. Analytical method  .........................................................................  26
3.3.5. Data processing method  ................................................................  26
CHAPTER 4: RESULTS AND DISCUSSION  .................................................  27
4.1.  The  results  of  selecting  the  optimal  conditions  for  the  extraction 
process ......................................................................................................  27
4.1.1. Choose the optimal solvent  ...........................................................  27
4.1.2. Choose the optimal salt concentration  ..........................................  33
4.1.3. Choose the optimal extraction time  ..............................................  36
4.1.4. Choose the ratio of algae biomass-solvent volume  ......................  38
4.2.  Survey  results  on  the  method  of  processing  C-PC  solution  into  dry 
powder ......................................................................................................  42 
v
4.2.1. Experiment results images  ............................................................  42
4.2.2. The quality comparison between freeze-drying method and natural 
drying  ......................................................................................................  43
4.2.3. Recovery efficiency of the refining process  .................................  44
CHAPTER 5: CONCLUSION AND SUGGESTION  .......................................  46
5.1. Conclusion  ..........................................................................................  46
5.2. Suggestion  ...........................................................................................  46
REFERENCES  ...................................................................................................  48
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