[Khóa luận tốt nghiệp] Investigation of a simple method for extraction c-phycocyanin from the blue-green microalgae arthrospira platensis
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CONTENTS
CHAPTER 1. INTRODUCTION ......................................................................... 1
1.1. Preface ................................................................................................... 1
1.2. Objectives ............................................................................................. 2
1.3. Requirements ........................................................................................ 2
CHAPTER 2. LITERATURE REVIEW .............................................................. 3
2.1. An overview of Arthrospira platensis .................................................. 3
2.1.1. Introduction about Arthrospira platensis ....................................... 3
2.1.2. Taxonomy of Arthrospira platensis ............................................... 4
2.1.3. Chemical composition of the algae Arthrospira platensis ............. 6
2.1.4. The applications of Arthrospira platensis ..................................... 9
2.1.4.1. For human health ................................ ................................ ................. 9
2.1.4.2. In the cosmetics industry ................................ ................................ . 10
2.1.4.3. Some studies show positive results ................................ ................. 10
2.2. An overview of C-phycocyanin .......................................................... 11
2.2.1. Introduction about C-phycocyanin ............................................... 11
2.2.2. Characteristics of C-phycocyanin ................................................. 12
2.2.3. Applications of C-phycocyanin .................................................... 13
2.2.4. Some studies on the use of C-phycocyanin .................................. 14
2.3. Studies on C-phycocyanin extract in the world and Vietnam ............ 18
2.3.1. Studies on C-phycocyanin extract in the World ........................... 18
2.3.2. Studies on C-phycocyanin extract in Vietnam ............................. 18
CHAPTER 3: MATERIALS AND EXPERIMENTAL METHODS ................ 20
iv
3.1. Materials ............................................................................................. 20
3.1.1. The origin ...................................................................................... 20
3.1.2. Place and time of experiment ....................................................... 20
3.2. Chemicals and equipments ................................................................. 20
3.3. Research methods ............................................................................... 21
3.3.1. Extraction method of C-PC .......................................................... 21
3.3.2. Factors affecting the extraction of C-PC ...................................... 23
3.3.3. Investigate the method of processing C-PC solution into dry
powder .................................................................................................... 24
3.3.3.1. Treatment of C-PC solution into dry powder by freeze-drying
method ................................ ................................ ................................ ................... 24
3.3.3.2. Treatment of C-PC solution into dry powder by natural
evaporation method ................................ ................................ .............................. 25
3.3.4. Analytical method ......................................................................... 26
3.3.5. Data processing method ................................................................ 26
CHAPTER 4: RESULTS AND DISCUSSION ................................................. 27
4.1. The results of selecting the optimal conditions for the extraction
process ...................................................................................................... 27
4.1.1. Choose the optimal solvent ........................................................... 27
4.1.2. Choose the optimal salt concentration .......................................... 33
4.1.3. Choose the optimal extraction time .............................................. 36
4.1.4. Choose the ratio of algae biomass-solvent volume ...................... 38
4.2. Survey results on the method of processing C-PC solution into dry
powder ...................................................................................................... 42
v
4.2.1. Experiment results images ............................................................ 42
4.2.2. The quality comparison between freeze-drying method and natural
drying ...................................................................................................... 43
4.2.3. Recovery efficiency of the refining process ................................. 44
CHAPTER 5: CONCLUSION AND SUGGESTION ....................................... 46
5.1. Conclusion .......................................................................................... 46
5.2. Suggestion ........................................................................................... 46
REFERENCES ................................................................................................... 48
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