ThiNganHang Sách - Tài liệu thi tuyển
30 câu trắc nghiệm Quản trị nguồn nhân lực (Có Đáp án)
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TABLE OF CONTENTS
COMMITMENT ............................................................................................................ i
ACKNOWLEDGEMENT ........................................................................................... iv
LIST OF TABLE ........................................................................................................... v
LIST OF FIGURE ........................................................................................................ vi
LIST OF ABBREVIATIONS ................................................................................... viii
ABTRACT ..................................................................................................................... ix
CHAPTER I. INTRODUCTION ................................................................................. 1
1.1. Introduction ........................................................................................................... 1
1.2. Objective ............................................................................................................... 2
1.2.1. Objective ............................................................................................................. 2
1.2.2. Specific objectives .............................................................................................. 2
CHAPTER II. LITERATURE REVIEWS ................................................................. 3
2.1. Duck status in the world. .......................................................................................... 3
2.2. The importance of duck in Vietnam. ........................................................................ 5
2.3. Diversity of Vietnamese duck .................................................................................. 6
2.4. Mitochondrial DNA as a tool for assessing the genetic diversity. ........................... 9
2.5. General information of the five duck breeds in this study ..................................... 12
2.5.1. General characteristics ......................................................................................... 12
2.5.2. Co duck ................................................................................................................ 12
2.5.3. Co Lung duck ..................................................................................................... 12
2.5.4. Na Tau duck......................................................................................................... 13
2.5.5. Troi duck.............................................................................................................. 14
2.5.6. Dom duck ............................................................................................................ 15
CHAPTER III. MATERIALS AND METHODS .................................................... 16
3.1. Material ................................................................................................................... 16
3.2. Place and time of study ........................................................................................... 17
3.2.1. Place of study ...................................................................................................... 17
3.2.2. Time of study ....................................................................................................... 17
3.3. Method .................................................................................................................... 18
3.3.1. Sample collection ................................................................................................ 18
iv
3.3.2. DNA isolation ...................................................................................................... 18
3.3.3. PCR amplification and sequencing ..................................................................... 20
3.3.4. Data Analysis ....................................................................................................... 21
CHAPTER IV. RESULTS AND DISCUSSION ....................................................... 23
4.1. Results of DNA extraction ..................................................................................... 23
4.2. Amplification of D-loop region and sequencing .................................................... 23
4.2.1. PCR results .......................................................................................................... 23
4.2.2. Sequence identification ....................................................................................... 24
4.3. Phylogenetic analysis and distribution of haplotypes of all 5 duck breeds in
Vietnam ............................................................................................................ 25
4.3.1. Polymorphism and genetic diversity ................................................................... 25
4.3.2. Distribution of haplotypes of 84 samples belonging to 5 duck breeds ............... 27
4.3.3. Phylogenetic analysis of 84 samples belonging to 5 duck breeds ....................... 29
4.4. Phylogenetic analysis and distribution of haplotypes of each breed ...................... 30
4.4.1. Phylogenetic analysis and distribution of haplotypes of Co breed ..................... 30
4.4.2. Phylogenetic analysis and distribution of haplotypes of Co Lung breed ............ 32
4.4.3. Phylogenetic analysis and distribution of haplotypes of Na Tau breed .............. 34
4.4.4. Phylogenetic analysis and distribution of haplotypes of Troi breed ................... 35
4.4.5. Phylogenetic analysis and distribution of haplotypes of Dom breed .................. 37
4.5. Discussion ............................................................................................................... 39
CHAPTER V. REFERENCES ................................................................................... 42
APPENDIX................................................................................................................... 44